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KMID : 0900920000240010049
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Korean journal of Animal Reproduction
2000 Volume.24 No. 1 p.49 ~ p.57
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Developmental Potentials of Clone Embryos Derived from Bovine Fetal Fibroblast Cells
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Cheong H.-T.
Kwon D.-J.
Park Y.-S.
Hwang H.-S.
Park C.-K.
Yang B.-K.
Kim C.-I.
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Abstract
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This study was conducted to investigate the developmental potential of cloned embryos derived from bovine fetal fibroblast cells, and the effect of quiescent treatment, passage number and origin of donor cells on in vitro development of cloned embryos. Fetal skin and liver-derived fibroblast cells were transferred to enucleated oocytes after serum starvation or nontreatment (cycling). After electrofusion. reconstituted embryos were activated with Ca^{＋＋}-ionophore and cycloheximide, and cocultured for 7~9 days with BRL cells. Some blastocysts were transferred to recipient cows 7~8 days post estrus. The development rate to the blastocyst stage of serum starved cell-derived embryos was higher (25.3%) than that of actively dividing cells-derived embryos (15.9%), The rates of blastocyst formation were 23.1~25.0% after transfer of cell passaged 4 to 6 times, and 23.8 and 25.2% after transfer of fetal skin and liver cells, respectively. After embryo transfer, 34.4% and 15.6% of recipient cows were pregnant on Day 60 and 120, respectively, and one male calf was produced from skin-derived vitrified blastocyst. The result of this study showed that the development of cloned embryos. was enhanced by quiescent treatment, but did not different among the cells passaged 4 to 6 times, and between skin and liver cells. This result also confirms that offspring can be obtained from the vitrified clone embryo derived from fetal skin cell.
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KEYWORD
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Nuclear transfer, Fetal fibroblast, Serum starvation, Clone bull
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